Saibyasachi Nath Choudhury

941, East Cherry Street

Appartment # 101 West

Missouri State University

Springfield

Missouri, 65807

United States of America

Objective:

To seek Research oriented Higher Education so that I can improve my subject knowledge further and apply them for the development of common people of our society.

Technical skills:

      Biotechnological

Biochemical & Instrumental methods

1.      Protein Purification & Estimation.

2.      Gel Electrophoresis

3.      Immobilization of Enzymes

4.      Chromatography Techniques

Immunological Techniques

1.      ELISA

2.      Precipitation

3.      Agglutination

4.      Immunodiffusion

Molecular Biological Techniques

1.      Isolation of Genomic DNA

2.      RAPD characterization of plant genomic DNA

3.      Blotting Techniques

4.      PCR amplification

Culture Techniques

                       Plant Tissue Culture.

      Computer skills 

MS-office,

MS-windows,

Internet

Genetic diversity Mapping Software (STASTICA-CLUSTER ANALYSIS)

Academic qualification:

Project/Dissertation:

·        RAPD CHARACTERIZATION OF OPEN POLLINATED PROGENY OF MALLIKA (2/8) MANGO (Mangifera indica L.)

Dissertation done as a part of the fulfillment of Masters Degree of Science in Biotechnology, under the guidance of Dr. P. Narayanaswamy, Associated Professor, Molecular Biology Laboratory (an ISO 9001 Lab.), Division of Horticulture, University of Agricultural Science (GKVK), Bangalore.

            Description of work:

The work is about preparing RAPD profile of open pollinated progeny (2/8) of Mallika mango. The specimen i.e. mature leaf is collected from the orchard of GKVK, UAS, Bangalore. The leaf is dried, powdered and DNA is extracted by CTAB method as given by Probeski with slight modification. The presence of extracted DNA is checked by running on 1.2% agarose gel. The extracted DNA is subjected to PCR amplification by using protocols of Williams et al. (1990), and Welsh and Mc Clelland (1990) with slight modification. The amplified DNA is again subjected to 1.2% agarose gel electrophoresis stained with Ethidium bromide along with 100 bp ladder marker to estimate the sizes of the DNA fragment. The DNA profiles obtained for 2/8 were manually scored along with the other profiles obtained in other progenies and a Dendrogram or Dissimilarity Matrix was developed using Squared Euclidean Distance and Clustering based on Wards method. The Dendrogram showed clearly the genetic dissimilarities between 12 progenies taken.

·        STUDY OF CHROMOSOMAL BANDING TECHNIQUE

Studied as a part of academic curriculum for M. Sc, Biotechnology, Previous year.

            Description of work:

The study of chromosome banding technique comprises of its evolution, its various types, and usefulness. Chromosome banding technique is the staining technique of chromosome for its identification by observing the differential banding pattern of chromosome. The evolution of banding technique from Ultramicrospectrophotometric procedure to the most modern technique i.e., by computerized procedures is studied.

The principle, background, chemical aspects, procedure, etc. of staining process of chromosome to get various types of bands are studied includes conventional staining techniques, Q-banding (with different stains), G-banding, C-banding, R-banding, T-banding, O-banding, N-banding, Hy-banding, Restriction enzyme banding.

The chromosome banding technique has various usefulness in modern day. It permits the visualization of molecular sequences at the cellular microscopic level,. Evolutionary relationship between different species can be found by observing banding patterns. The related species can be identified by identifying the isochores of the chromosomes by banding technique. Comparative studies of different banding techniques in chromosomes of different species were found effective and useful in identification of translocation of chromosomes. By this technique, the chromosome aberration of the abberated chromosome can be identified which is very useful for the treatment of patients suffering from chromosomal disorder.

·        A SURVEY OF WATER MICROFLORA OF RIVER BARAK, ASSAM.

Dissertation done as a part of fulfillment of Bachelors degree of science with honors in Botany during the third year of three year degree course.

          Description of work:

The project is about quantitative analysis of Bacteria and Fungi, and qualitative analysis of Fungi in pre- and post- treated water of river Barak. Two water samples were collected, one from river Barak (i.e., pre-treated water) and other from the Silchar Municipal Water Treatment Plant i.e., the post treated water.

For the growth of colonies, two media i.e., PDA and NA media were used. Serial dilution of the water sample was made in multiples of ten, and then inoculated in the culture plate by pour-plate method. The inoculated culture plate is then incubated at 25-35˚C for 48 hours. After the growth of colonies, colony counting is done manually by Bacteriological colony counter and the number of microorganism per ml. of water inoculated is calculated by standard formula. From the investigation it was concluded that the number of fungal population exceeds the Bacterial population, and two Fungal species (Rhizopous and Rhizopodium) appeared from post-treated water sample, but not from pre-treated sample of water.

Other activities:

                                                                                               Yours sincerely

                                                                                    Saibyasachi Nath Choudhury